Reproduction Abstracts

In recent years, advances in molecular biology and reproductive engineering make generation of knockout (KO) animals possible. KO animals are useful and powerful tools for agriculture as well as biomedical research. Zinc finger nuclease (ZFN) and transcription activator-like effector nuclease (TALEN) genome editing technology enables site directed engineering of the genome. The aim of the present study was to ZFN and TALEN system could work on editing genome of cultured porcin...

The aim of the present study was to evaluate fertility of vitrified rat matured-oocytes with surrounding cumulus cells after IVF with cryopreserved sperm. Oocytes with surrounding cumulus cells and cumulus cells denuded oocytes were vitrified with 30% (v/v) ethylene glycol+0.5 M sucrose+20% (v/v) FCS in PBS (PB1) by using Cryotop. After warming, oocytes were co-cultured with fresh or cryopreserved epididymal sperm for 10 h. Although the fertilization (two pronuclei formation: ...